Galaxy S6 Edge :: Can Disabling App Have Negative Effect On Battery

I know a lot of you here would love the battery life i am getting so i do not mean to sound like i am complaining! I am mostly just curious! Basically i was amazed with how good my battery life was initially. I think on my second full charge i managed 8h30mins screen on time and probably averaged somewhere between 7hours - 8h30mins for the fist few days (needless to say i made tweaks to achieve those results as i always do when i buy a new phone)

I have since disabled anther 2 or 3 apps and i have also turned off location and used black themed wallpapers purely just to test if i could get even further from the battery! However the opposite has happened! Suddenly iam doing well if i make it to 5h30mins

I know that may not seem bad .......... but its not great considering the amount of tweaks i have made and i am at a loss to understand how i was getting 7-8 hours screen on time for the first few days/week!

Iam wondering if i could have disabled an app that could be behind this?

When i viewed my battery usage before i would often see chrome, facebook etc using a little battery where as now its screen, android system, android os, cell standby, device idle etc and recently peoplestripe is appearing as using 1%...